ecg ad instruments powerlab 8 30 recorder with bioamp electrodes (ADInstruments)
Structured Review

Ecg Ad Instruments Powerlab 8 30 Recorder With Bioamp Electrodes, supplied by ADInstruments, used in various techniques. Bioz Stars score: 98/100, based on 3549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 3549 article reviews
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1) Product Images from "Organ-specific translation elongation rates measured by in vivo ribosome profiling"
Article Title: Organ-specific translation elongation rates measured by in vivo ribosome profiling
Journal: bioRxiv
doi: 10.1101/279257
Figure Legend Snippet: Experimental setup. (A) Layout of mouse organs susceptible to translation inhibitors. Organs marked in green are permeable for both harringtonine and cy-cloheximide within 15 sec post-injection. Translation elongation rate can be directly measured in these organs. Testes (shown in yellow) are partially permeable, and brain is mostly unaffected by inhibitors. (B) High doses of harringtonine severely drop heartbeat rate. We established that 2.5 mg/ml had no effect on the beat rate, whereas 5 mg/ml decreased it twice. 10 mg/ml caused cardiac arrest in most of the cases. All injections were done in a tail vein - injection volume was 200 μ l and the weight of mice was 20-25 g. (C) Representative electrocardiograms (ECG) from injected mice. The upper track shows the slowdown of the beat rate in response to 5 mg/ml harringtonine. The lower track shows no difference after the injection of 2.5 mg/ml harringtonine. The effect of harringtonine appears roughly 20 sec after the injection. The red line marks the injection event.
Techniques Used: Injection
Figure Legend Snippet: Measuring translation elongation rate in vivo . (A) ECG monitoring of heart function before and after drug injection to ensure the heart beat rate is unaffected at the chosen dosage. (B) Experimental design for a time-resolved study. (C) Linear regression analysis of injection timepoints to infer the elongation rate. (D) Comparison of mean translation elongation rates in three organs. (E) Metagene analysis of ribosome profiling sequences. Genes longer than 2,000 nucleotides are presented. The first 1,500 nucleotides are shown starting from the first codon of the reading frame. Solid lines represent mean signal from biological replicates, and light shading corresponds to a standard deviation.
Techniques Used: In Vivo, Injection, Comparison, Standard Deviation